Translational_Unit

Part:BBa_K1641017

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-11)


Up-side-down RBS::mcherry-ssra with Vox, Vika recognition sites

This is a special RBS::mcherry-ssra inverted into up-side-down form, with two Vox sites at the upstream and downstream of the gene. Vox can be recognized and the middle sequence can be restored by invertase Vika (BBa_K1641003). Ssra-tag in this sequence is moderately fast type (BBa_M0052) that accelerates the degradation but at a speed far less than expression.

This part can be used to construct the reporter for real-time invertase dynamics analysis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None